Sukanya P and Lingayya Hiremath
The roots of Hemidismus indicus have been used in traditional and indigenous system of medicines thought the humankind for thousands of years. The plant Hemidismus indicus enriched with many phyto-constituents, which are responsible to cure many ailments. The present study was aimed to physiochemical screening and estimation of the bioactive compounds by using advance analytical tools. The study results in the qualitative and quantative estimation of the secondary metabolites; which are industrially important in standardization of the herbal drug products. The heavy metals and residues are the main intimidation in herbal drug formulations. The advance analytical instruments like HPLC and ICPMS are used in the estimation of Aflotoxins and Heavy metals in the study. The validated analytical methods and tools are applied here for the identification and quantifications of the Hemidismus indicus root powder. The same method and assay can be used as quality control parameters in standardization of the plant extracts and herbal drug formulations. The developed HPTLC method is found to be sensitive, specific, precise and accurate for the identification and quantification of isovanillin, rutin and coumarin in Hemidismus indicus. The finger prints derived from the HPTLC can be used as the athentification tool for the Hemidismus indicus roots.
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