International Journal of Pharmaceutical and Clinical Research

International Journal of Pharmaceutical and Clinical Research

Vol. 3, Issue 1, Part A (2021)

Preparation and physicochemical characterization of novel anticancer ck-10 loaded nanoparticles to check biodegradation, compatibility and stability

Author(s):

Girgis Samuel, Uddin Nazim, Ankur Sharma

Abstract:
The main objective of this project was to formulate novelamphiphilic PLGA nanoparticles having better physicochemical properties for the delivery of the novel peptide (CK-10) to the cancerous/tumour tissue. Double emulsion/Solvent evaporation [DE/SE] and a novel microfluidic techniques were used to formulate the nanoparticles. Size & images were characterized by laser obscuration time whereas invitro release were measured by modified Lowry assay. Stability was checked by high performance liquid chromatography and capillary zone electrophoresis. Evaluation of the compatibility, interaction and shelf life stability were confirmed by FTIR and differential scanning calorimeter. Water absorption and its associated changes in the physicochemical properties were measured by various color indicator and potentiometric titrations techniques. PLGA/Poloxamer & PLGA/╬▓Cyclodextrin nanoparticles produced by the novel microfluidic technique showed the highest in-vitro release with good size criteria and optimum biodegradation for the CK10 delivery to the cancer cells. Better physicochemical properties for the CK-10 loaded PLGA nanoparticles could be obtained by using the novel microfluidic technique to improve the RAN blocking by CK-10.

Pages: 01-12  |  1661 Views  332 Downloads

How to cite this article:
Girgis Samuel, Uddin Nazim, Ankur Sharma. Preparation and physicochemical characterization of novel anticancer ck-10 loaded nanoparticles to check biodegradation, compatibility and stability. Int. J. Pharm. Clin. Res. 2021;3(1):01-12.