Vol. 6, Issue 2, Part A (2024)

This study focuses on the phytochemical analysis and antimicrobial efficacy of Cassia tora leaves extract, a plant known for its medicinal properties. The research aims to identify the bioactive compounds present in the leaves and evaluate their antimicrobial activity using the National Committee for Clinical Laboratory Standards (NCCLS) antimicrobial susceptibility method. The phytochemical screening of the Cassia tora leaves extract was conducted, revealing the presence of alkaloids, flavonoids, tannins, saponins, and glycosides. These compounds are recognized for their potential antimicrobial properties. The extract was prepared using methanol as the solvent and subjected to antimicrobial testing against three microbial strains: Gram-positive Staphylococcus aureus, Gram-negative Escherichia coli, and the fungal strain Candida albicans. Antimicrobial susceptibility was assessed by measuring the zone of inhibition around the extract and determining the minimum inhibitory concentration (MIC) following NCCLS guidelines. The results demonstrated that the Cassia tora leaves extract exhibited significant antimicrobial activity, particularly against Staphylococcus aureus and Candida albicans, while showing moderate activity against Escherichia coli. The observed antimicrobial effects can be attributed to the synergistic action of the various phytochemicals present in the extract. In conclusion, the findings confirm the traditional medicinal use of Cassia tora and suggest its potential as a source of natural antimicrobial agents. Further research is recommended to isolate specific compounds and explore their individual antimicrobial mechanisms.